A Multiplexed Fluorescent Calcium and NFAT Reporter Gene Assay to Identify GPCR Agonists
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چکیده
منابع مشابه
A Multiplexed Fluorescent Calcium and NFAT Reporter Gene Assay to Identify GPCR Agonists
Intracellular calcium response and resulting calcium signaling to an agonist-GPCR interaction are important for the measurement of compound activity in the GPCR drug development. The increase in cytosol calcium concentration can be measured by the fluorescent calcium indicator dye such as Fluo-4 in a quick assay (in 3-5 minutes) using the fluorescence imaging plate reader. The calcium signaling...
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ژورنال
عنوان ژورنال: Current Chemical Genomics and Translational Medicine
سال: 2013
ISSN: 2213-9885
DOI: 10.2174/2213988501307010001